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Image Search Results
Journal: Biochimica et biophysica acta
Article Title: Development of a homogeneous AlphaLISA ubiquitination assay using ubiquitin binding matrices as universal components for the detection of ubiquitinated proteins.
doi: 10.1016/j.bbamcr.2012.03.018
Figure Lengend Snippet: Fig. 5. E3 universality. A) SUMO-Hrd1 (RING E3) was titrated in 2 fold dilutions from 20 nm to .031 nM and autoubiquitination activity (10 nM E1, 250 nM E2 Ubch5b and 250 nM ubiquitin) was measured up to 80 min. B) GST-Smurf1 (HECT E3) was titrated in 2 fold dilutions from 5 nM to .031 nM and autoubiquitination activity (10 nM E1, 250 nM E2 Ubch7 and 250 nM ubiquitin) up to 120 min. C) GST-Traf6 (RING E3) was titrated in 2 fold dilutions from 20 nm to 0.031 nM and autoubiquitination activity (10 nM E1, 100 nM E2 Uev1A/ Ubc13 and 500 nM ubiquitin) was measured up to 80 min.
Article Snippet: Mdm2 mouse monoclonal antibody (smp14) was ordered from Abcam (Cambridge, MA), Hrd1 rabbit polyclonal antibody (C-term) from Abgent, Inc. (San Diego, CA),
Techniques: Activity Assay, Ubiquitin Proteomics